Description / Human holo-Tranferrin (Animal free) protein
Transferrin (TF) is found in vertebrate that mediates the transport of iron through blood plasma. When not bound to iron, the protein is known as apo-Transferrin. Each molecule contains two binding sites for iron in the ferric form Fe(III). The iron-bound holo-Transferrin (HTF) can bind to the dimeric Transferrin receptor (TfR) on a cell’s surface and is subsequently internalized by endocytosis. A reduction of pH in the vesicle decreases its iron-affinity and causes HTF to release bound iron ions. Transferrin appears to be essential for most cells growing in tissue culture. ReliaTech's Recombinant Human holo-Transferrin is a 76 kDa glycoprotein consisting of 679 amino acid residues.
More Information
| Size | 1000 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | The biological activity is determined by the stimulation of cell proliferation of MDCK cells in serum-free cell culture medium. |
| Purity Confirmation | ≥ 98% by SDS-PAGE |
| Length [aa] | 679 |
| Molecular Weight | 76 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Centrifuge the vial prior to opening. Do not vortex. The lyophilized HTF should be reconstituted in water to a concentration of 1.0 mg/mL. |
| Stability and Storage | The lyophilized HTF is stable at room temperature for 1 month and at 2 °C to 8 °C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Application | cell culture |
| Synonyms | HTF, Serotransferrin, Transferrin, TF, Holo Transferrin, Beta-1 metal-binding-globulin, Siderophilin |
| Uniprot ID | P02787 |
| Protein RefSeq | NP_001054.2 |
| mRNA RefSeq | NM_001063.4 |