Description / anti- His-tag polyclonal antibody
The His-tag antibody was generated by immunization of rabbits with several recombinant His-tagged proteins produced in insect cells and E. coli. It was antigen-affinity purified using a His-tagged protein as matrix.
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| Size | 50 µg |
|---|---|
| Source | Rabbit |
| Biological Activity | Western Blot: Use 1-5 µg/ml |
| Clone Nr. | Rabbit IgG |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | The anti-His-tag antibody was generated by immunization of rabbits with different highly purified His-tagged proteins produced in insect cells. The anti-His-tag antibodies were then purified from sera by antigen-affinity chromatography using a His-peptide |
| Application | WB |
| Synonyms | His-tag |