Description / anti-mouse HGF Activator Inhibitor Type 1 (HAI-1) monoclonal antibody
Hepatocyte growth factor activator inhibitor1 (HAI1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGF-A) and matripas. The membraneanchored HAI1 consists of two Kunitz domains, a LDL-recepto-rlike domain, and a C-terminal transmembrane domain. Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface. The shedding of HAI1 and HGFA/HAI1 complex is enhanced by treatment with phorbol 12myristate, 13acetate or IL1β. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#3G21) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of HAI-1extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity |
| Antigen | recombinant mouse HAI-1 extracellular domain |
| Application | WB |
| Synonyms | Spint1; HAI-1 |
| Uniprot ID | Q9R097 |
| Protein RefSeq | NP_058603.2 |
| mRNA RefSeq | NM_016907.3 |