Description / anti-human HAI-1 monoclonal antibody
HAI1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase. The membrane anchored HAI1 consists of two Kunitz domains, a LDL receptorlike domain, and a C-terminal transmembrane domain. Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface . The shedding of HAI1 and HGFA/HAI1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL1β. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Clone Nr. | (#3X89) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of HAI-1 extracellular domain. |
| Antigen | recombinant human HAI-1 EC domain |
| Application | Capture Ab |
| Synonyms | SPINT1; HAI; HAI1; MANSC2 |
| Uniprot ID | O43278 |
| Protein RefSeq | NP_857593.1 |
| mRNA RefSeq | NM_181642.2 |