Description / human GASP-1 protein
Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66 kDa by SDS-PAGE analysis under non-reducing conditions.
More Information
| Size | 5 µg |
|---|---|
| Source | CHO cells |
| Biological Activity | Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 µg/ml in the presence of 5ng/ml of human Myostatin (GDF-8). |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 542 |
| Molecular Weight | 55-66 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | LPPIRYSHAG ICPNDMNPNL WVDAQSTCRR ECETDQECET YEKCCPNVCG TKSCVAARYM DVKGKKGPVG MPKEATCDHF MCLQQGSECD IWDGQPVCKC KDRCEKEPSF TCASDGLTYY NRCYMDAEAC SKGITLAVVT CRYHFTWPNT SPPPPETTMH PTTASPETPE LDMAAPALLN NPVHQSVTMG ETVSFLCDVV GRPRPEITWE KQLEDRENVV MRPNHVRGNV VV |
| Synonyms | GDF-associated serum protein-1, GASP, KIAA0443, WFIKKNRP |
| Uniprot ID | Q5JY77 |
| Protein RefSeq | NP_055525.3 |
| mRNA RefSeq | NM_014710.4 |