Description / anti-mouse Galectin-7 monoclonal antibody
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#8J52) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant Galectin-7. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. |
| Antigen | Recombinant mouse Galectin-7 |
| Application | WB, IHC |
| Synonyms | Lgals7; Pig1; Galectin-7 |
| Uniprot ID | O54974 |
| Protein RefSeq | NP_032522.2 |
| mRNA RefSeq | NM_008496.4 |