Description / anti-human G-CSFR (CD114) monoclonal antibody
Human G-CSF R is 836 amino acids (aa) in length, with a 23 aa signal sequence, a 604 aa extracellular region, a 26 aa transmembrane segment, and a 183 aa cytoplasmic domain. As a result of alternative splicing, at least four isoforms of G-CSF R exist that differ in their transmembrane C-terminal region. Human and mouse G-CSF R are 64% identical at the aa level. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Clone Nr. | (#8F19) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of G-CSF-R extracellular domain (also called CD114). |
| Antigen | recombinant human G-CSF-R EC domain |
| Application | WB, FC |
| Synonyms | CSF3R; CD114; GCSFR |
| Uniprot ID | Q99062 |
| Protein RefSeq | NP_000751.1 |
| mRNA RefSeq | NM_000760.3 |