Flt-3 Ligand Anti-Human 100 µg

In stock

Cat-Nr.
101-M420
Size
100 µg
  €533.00

Description / Flt-3 Ligand

Flt-3 Ligand, also known as FL, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a non-covalently linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt -3 Ligand also generates membraneassociated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt3/ FlK-1. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation.

More Information

Size 100 µg
Source Mouse
Isotype IgG1
Clone Nr. (#9G23)
Species Reactivity Human
Formulation lyophilized
Buffer PBS
Reconstitution Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and Storage Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of Flt-3 ligand.
Antigen Recombinant human Flt-3 ligand
Application WB, N
Synonyms FLT3LG; FL; FLT3L
Uniprot ID P36888
Protein RefSeq NP_004110.2
mRNA RefSeq NM_004119

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