Description / anti-human Enteropeptidase monoclonal antibody
Enteropeptidase/Enterokinase initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The multi-domain-containing the heavy chain consists of a short cytoplasmic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2 |
| Biological Activity | Western Blot: Use at 250 ng/ml-1 µg/ml |
| Clone Nr. | (#8S12) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human recombinant Enteropeptidase (also called Enterokinase). The IgG fraction of culture supernatant was purified by Protein G affinity chromat |
| Antigen | human recombinant Enteropeptidase (also called Enterokinase) |
| Application | WB |
| Synonyms | TMPRSS15; ENTK; PRSS7; Enterokinase; Serine protease 7; Transmembrane protease serine 15 |
| Uniprot ID | P98073 |
| Protein RefSeq | NP_002763.2 |
| mRNA RefSeq | NM_002772.2 |