Description / human DLL-1, soluble protein
Human sDLL-1 comprises the extracellular signaling domain of DLL1, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors. It is expressed in the heart and pancreas, and to a lesser extent in various other tissues. DLL-1 functions to specifically activate the Notch-1 and Notch-2 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. DLL-1 suppresses differentiation of hematopoietic progenitor cells into the B-cell lineage while promoting differentiation to T-cell and NK cell precursors. Recombinant human sDLL-1 is a 57-60 kDa glycoprotein containing 522 amino-acid residues.
More Information
| Size | 25 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 μg/ml. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 522 |
| Molecular Weight | 57-60 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS, pH 7.5 |
| Protein Sequence | SGVFELKLQE FVNKKGLLGN RNCCRGGAGP PPCACRTFFR VCLKHYQASV SPEPPCTYGS AVTPVLGVDS FSLPDGGGAD SAFSNPIRFP FGFTWPGTFS LIIEALHTDS PDDLATENPE RLISRLATQR HLTVGEEWSQ DLHSSGRTDL KYSYRFVCDE HYYGEGCSVF CRPRDDAFGH FTCGERGEKV CNPGWKGPYC TEPICLPGCD EQHGFCDKPG ECKCRVGWQG RY |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | DLL1; DL1; Delta; DELTA1 |
| Uniprot ID | O00548 |
| Protein RefSeq | NP_005609 |
| mRNA RefSeq | NM_005618 |