Description / anti-human CEACAM-1 monoclonal antibody
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, it forms noncovalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1, kappa |
| Biological Activity | ELISA: Use at 10 µg/ml; WB: Use at 5 µg/ml; Immunohistochemistry: Use at 10 µg/ml; FACS analysis: Use at 10 µg/ml. |
| Clone Nr. | (#B3) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | Monoclonal antibodies were produced with the help of NS1/0 mice using recombinant human soluble CEACAM-1-Fc lacking the N-domain (produced in HEK293 cells) as the immunizing antigen. The antibody (clone #B3) was purified by Protein G chromatogra |
| Antigen | recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells) |
| Application | WB, IHC, E, IF, IP, FC |
| Synonyms | CEACAM1; BGP; BGP1; BGPI, CD66a |
| Uniprot ID | P13688 |
| Protein RefSeq | NP_001192273.1 |
| mRNA RefSeq | NM_001205344.1 |