Description / CD80
B7-1(CD80) and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the downregulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be upregulated through interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily.
More Information
Size | 100 µg |
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Source | Mouse |
Isotype | IgG1 |
Clone Nr. | (#6N36) |
Species Reactivity | Human |
Formulation | lyophilized |
Buffer | PBS |
Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of CD 80 (also called B7-1). |
Antigen | Human recombinant CD80 EC domian |
Application | IHC, FC, N |
Synonyms | CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1 |
Uniprot ID | P33681 |
Protein RefSeq | NP_005182.1 |
mRNA RefSeq | NM_005191.3 |