Description / anti-mouse CD40 Ligand monoclonal antibody
CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site. CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes. Both membrane bound and soluble CD40L induce similar effects on B cells.
More Information
| Size | 100 µg |
|---|---|
| Source | Hamster |
| Isotype | IgG2 |
| Clone Nr. | (#MAB0712) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a Armenian Hamster) immunized with mouse Th1 cells. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Mouse Th1 cells |
| Application | IHC, FC |
| Synonyms | Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5 |
| Uniprot ID | P27548 |
| Protein RefSeq | NP_035746.2 |
| mRNA RefSeq | NM_011616.2 |