Description / anti-human CD40 Ligand polyclonal antibody
CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site. CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes. Both membrane bound and soluble CD40L induce similar effects on B cells.
More Information
| Size | 100 µg |
|---|---|
| Source | Goat |
| Clone Nr. | Goat IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (human sCD40L). Anti-hsCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix. |
| Antigen | Recombinant Human sCD40 Ligand |
| Application | ELISA, WB |
| Synonyms | CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L |
| Uniprot ID | P29965 |
| Protein RefSeq | NP_000065.1 |
| mRNA RefSeq | NM_000074.2 |