Description / Anti-Mouse CD28 monoclonal antibody
CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Mouse CD28 is expressed constitutively on virtually 100% of mouse Tcells and on developing thymocytes.
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| Size | 50 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#17E9) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a Golden Syrian Hamster immunized with recombinant mouse CD28 protein). IgG fraction of the culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Recombinant mouse CD28 |
| Application | WB |
| Uniprot ID | P31041 |
| Protein RefSeq | NP_031668.3 |
| mRNA RefSeq | NM_007642.4 |