BMP-3 Anti-Human 100 µg

In stock

Cat-Nr.
101-M234
Size
100 µg
  €533.00

Description / BMP-3

BMP3, also known as osteogenin, the most abundant BMP in adult bone, is one of at least 15 structurally and functionally related BMPs, which are members of the TGFβ superfamily (13). BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs also regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types. Similar to most other TGFβfamily proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and rat BMP3 are 98% identical. BMP3 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. Biologically active BMP3 is a disulfidelinked homodimer of the carboxyterminal 110 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond (4). The role of BMP3 in bone is contradictory since, unlike osteogenin purified from bone, recombinant BMP3 has not shown osteogenic function (5). Several studies indicate that BMP3 is an inhibitor of osteogenic BMPs. BMP3 dorsalizes Xenopus embryos, the opposite effect of BMP2 or 4, which cause ventralization. BMP3 inhibits alkaline phosphatase production and induction of osteoblastic target genes in undifferentiated mesenchymal and osteogenic cell lines that have been treated with BMP2. BMP3 also induces the expression of TGFβ/ activin responsive genes, but not BMPresponsive genes.

More Information

Size 100 µg
Source Mouse
Isotype IgG2
Clone Nr. (#6J24)
Species Reactivity Human
Formulation lyophilized
Buffer PBS
Reconstitution Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and Storage Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of Bone Morphogenetic Protein 3 (BMP-3).
Antigen recombinant human BMP-3
Application WB
Synonyms BMP3; BMP-3A
Uniprot ID P12645
Protein RefSeq NP_001192
mRNA RefSeq NM_001201

All prices plus VAT + possible delivery charges