Description / anti-human BDNF polyclonal antibody
BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.
More Information
| Size | 100 µg |
|---|---|
| Source | Rabbit |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human BDNF (human Brain-Derived Neurotrophic Factor). Anti-human BDNF specific antibody was purified by affinity chromatography employing immobilized human BDNF matrix. |
| Antigen | E.coli derived Recombinant Human BDNF |
| Application | ELISA, WB, N, IHC |
| Synonyms | BDNF |
| Uniprot ID | P23560 |
| Protein RefSeq | NP_733931.1 |
| mRNA RefSeq | NM_170735.5 |