human BAFF Receptor (Fc-Tag, AVI-Tag), Biotinylated protein Human 20 µg
Description / human BAFF Receptor (Fc-Tag, AVI-Tag), Biotinylated protein
B-cell-activating factor receptor (BAFF-R), also known as Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C) and CD268 antigen, is a member of the TNFR superfamily. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis. BAFF-R is a type III transmembrane protein that binds with high specificity to BAFF (TNFSF13B) and initiates signalling pathways, which are critical for B cell maturation and survival. BAFF-R is highly expressed in spleen, lymph node, resting and activated B cells, resting CD4+ cells and peripheral blood leukocytes. Abnormally high levels of BAFF-R may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. Recombinant Human BAFF Receptor (Fc-Tag, AVI-Tag), Biotinylated consists of 324 amino acid residues, including a C-terminal AVI-tagged human Fc-Tag and has a molecular weight of approximately 44-52 kDa. The expressed protein was biotinylated in vivo by the BirA enzyme.
More Information
| Size | 20 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | Immobilized human BAFF at 2 μg/mL (100 μL/well) binds human BAFF receptor hFc & AVI, Biotinylated. The EC50 for this effect is 40-200 ng/mL. |
| Purity Confirmation | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
| Length [aa] | 324 |
| Molecular Weight | 44-52 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS (pH 7.4), may vary depending on the Lot |
| Protein Sequence | A DNA sequence encoding the human BAFF receptor (NP_443177.1)(Arg2-Ala71) was expressed with a C-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase. |
| Reconstitution | see Datasheet |
| Stability and Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Synonyms | B-cell-activating factor receptor, TNFRSF13C, Tumor necrosis factor receptor superfamily member 13C, BAFFR, CD268, CVID4, BAFF-R, BROMIX, prolixin, BLyS Receptor 3 |
| Uniprot ID | Q96RJ3 |
| Protein RefSeq | NP_443177.1 |
| mRNA RefSeq | NM_052945.4 |
| Endotoxin | < 0.1 ng/µg of protein (< 1 EU/µg) |