mouse Artemin protein Mouse 100 µg
Description / mouse Artemin protein
Artemin is a disulfide‐linked homodimeric neurotrophic factor structurally related to GDNF, neurturin and persephin. These proteins belong to the cysteine knot superfamily of growth factors that assume stable dimeric protein structures. Artemin, GDNF, persephin and neurturin all signal through a multicomponent receptor system, composed of RET (receptor tyrosine kinase) and one of the four GFRα (α1‐α4) receptors. Artemin prefers the receptor GFRα3‐RET, but will use other receptors as an alternative. Artemin supports the survival of all peripheral ganglia, such as sympathetic, neural crest and placodally‐derived sensory neurons, and dopaminergic midbrain neurons. The functional human artemin ligand is a disulfide‐linked homodimer of two 12.0 kDa polypeptide monomers. Each monomer contains seven conserved cysteine residues, one of which is used for interchain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine‐knot configuration. Recombinant Murine Artemin is a disulfide‐linked homodimer formed by two identical 113 amino acid subunits with a calculated molecular weight of 24.2 kDa.
More Information
| Size | 100 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | Immobilized recombinant mouse GFR Alpha-3/GFRA3 at 2 μg/mL (100 μL/well) binds recombinant mouse Artemin. The EC50 for this effect is 3-9 ng/mL. |
| Purity Confirmation | > 90 % as determined by SDS-PAGE. |
| Length [aa] | 373 |
| Molecular Weight | 40.6 kDa |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS (pH 7.4), may vary depending on the Lot |
| Protein Sequence | A DNA sequence encoding the mouse Artemin (NP_033841.1) (Ala112-Gly224) was expressed with the Fc region of human IgG1 at the N-terminus. |
| Reconstitution | see Datasheet |
| Stability and Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Synonyms | ART, ARTN, Enovin, Neublastin |
| Uniprot ID | Q9Z0L2 |
| Protein RefSeq | NP_001271120.1 |
| mRNA RefSeq | NM_001284191.1 |
| Endotoxin | < 0.1 ng/µg of protein (< 1 EU/µg) |