Arginine Deiminase (ADI) Mycoplasma 5 µg

In stock

Cat-Nr.
100-404S
Size
5 µg
  €112.00

Description / Arginine Deiminase (ADI)

Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.

More Information

Size 5 µg
Source E. coli
Biological Activity Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.
Purity Confirmation > 97% by SDS-PAGE & HPLC analyses
Length [aa] 409
Molecular Weight 46.3 kDa
Formulation lyophilized
Buffer 2mM Sodium Phosphate, pH 7.5 + 0.2mM DTT
Protein Sequence SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSAILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK DKLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKTDLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RETLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGVSE RT
Reconstitution Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri
Stability and Storage The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Synonyms ADI
Uniprot ID P23793
Protein RefSeq CAA38080.1
mRNA RefSeq AF182646.1

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