Description / Arginine Deiminase (ADI)
Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.
More Information
Size | 20 µg |
---|---|
Source | E. coli |
Biological Activity | Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml. |
Purity Confirmation | > 97% by SDS-PAGE & HPLC analyses |
Length [aa] | 409 |
Molecular Weight | 46.3 kDa |
Formulation | lyophilized |
Buffer | 2mM Sodium Phosphate, pH 7.5 + 0.2mM DTT |
Protein Sequence | SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSAILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK DKLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKTDLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RETLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGVSE RT |
Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
Synonyms | ADI |
Uniprot ID | P23793 |
Protein RefSeq | CAA38080.1 |
mRNA RefSeq | AF182646.1 |