Description / human ApoA-I protein
ApoA-I is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the “good cholesterol.” The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases. Recombinant human ApoA-I is a 28.2 kDa protein of 244 amino acid residues.
More Information
| Size | 100 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized Human ApoAI at 10 μg/mL (100 μL/well) binds biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 0.37 μg/mL. |
| Purity Confirmation | ≥ 95 % as determined by SDS-PAGE. |
| Length [aa] | 481 |
| Molecular Weight | 55 kDa (Monomer) |
| Species Reactivity | Hamster, Human |
| Formulation | lyophilized |
| Buffer | PBS + 10% Glycerol (pH 7.4), may vary depending on the Lot. |
| Protein Sequence | A DNA sequence encoding the pro-form of human APOA1 (CAA26097.1) (Met 1-Gln 267) was fused with Fc region of human IgG1 at the C-terminus. |
| Reconstitution | see Datasheet |
| Stability and Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Synonyms | APOA1, Apolipoprotein A-I |
| Uniprot ID | P02647 |
| Protein RefSeq | NP_000030.1 |
| mRNA RefSeq | NM_000039.1 |