Description / Anti-Mouse ALS monoclonal antibody
ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules. It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGFI. This complex increases the half-life of IGFI and retains it in the circulation for use in multiple tissues. Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603). It contains twentyone 20-23 aa LRRs that participate in proteinprotein interactions. There are two potential isoform variants that show N-terminal extensions. One contains a 66 aa substitution for aa 1 -5, while another possesses an 89 aa substitution again for aa 15. Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG1 |
| Clone Nr. | (#4K23) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of ALS. |
| Antigen | recombinant protein of ALS |
| Application | WB |
| Synonyms | Igfals; ALS; Albs; mKIAA4111 |
| Uniprot ID | P70389 |
| Protein RefSeq | NP_032366.2 |
| mRNA RefSeq | NM_008340.3 |