Description / human 4-1BBL protein
4-1BBL, a member of the TNF superfamily, is expressed in B cells, dendritic cells, activated T cells and macrophages. 4-1BBL binds to its receptor 4-1BB, and provides a co-stimulatory signal for T cell activation and expansion. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain. The soluble form of 4-1BBL contains the TNF-like portion of the extracellular domain of 4-1BBL. Recombinant human 4-1BBL is a soluble 19.5 kDa protein consisting of 185 amino acid residues.
More Information
| Size | 100 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | Immobilized Recombinant Mouse 4-1BB / TNFRSF9 / CD137 Protein at 2 μg/mL (100 μL/well) binds Recombinant Mouse 4-1BBL / TNFSF9 Protein, the EC50 is 2-6 ng/mL.NOTE: Results may vary with different PBMC donors. |
| Purity Confirmation | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
| Length [aa] | 466 |
| Molecular Weight | 51.4 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS (pH 7.4), may vary depending on the Lot |
| Protein Sequence | A DNA sequence encoding the Mouse 4-1BBL (NP_033430.1) (Arg104-Glu309) was expressed with the Fc region of human IgG1 at the N-terminus. |
| Reconstitution | see Datasheet |
| Stability and Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Synonyms | TNFSF9; CD137L; 4-1BB-L |
| Uniprot ID | P41273 |
| Protein RefSeq | NP_003802.1 |
| mRNA RefSeq | NM_003811.3 |