Bovine ECGFpro1 + Heparin (complete for blood ECs)

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Cat-Nr.300-090H-B
Size6 mg
Price105 €
SourceCattle
Formulationlyophilized (freeze-dried)
PurificationCrude extract
Biological ActivityThe working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal.
Species Reactivityhuman, mouse, rat, cattle, pig
Bufferwater
ReconstitutionReconstitute the contents of the vial in 2 ml of prewarmed (37 °C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.
Stability and StorageAlso stable at 4 °C for several weeks it is recommended to store the product below 0 °C. After reconstitution the product shout be stored in aliquots at -20 °C to -70 °C.
Application RemarksCan be used for blood endothelial cell cultivation.
SynonymsEndothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS)
DescriptionEndothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate. ECGFpro1 is supplemented with recombinant human VEGF165 (corresponding to 10ng/ml) a concentration sufficient for the cultivation of blood vascular endothelial cells like HUVEC and HMVEC. Endothelial cells from human umbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows cultivating endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.

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