Human IL-4 receptor alpha (CD124), soluble

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Cat-Nr.S01-029S
Size5 µg
Price75 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & HPLC analyses
Length [aa]209
Molecular Weight23.9 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityThe ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of IL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
Synonymssoluble Interleukin-4Rα, soluble IL-4 Receptor alpha, CD124
DescriptionIL-4 can signal through type I and type II receptor complexes, which share a common gamma chain. The type I receptor contains, in addition to the gamma chain, an IL-4R alpha subunit, whereas the type II receptor contains the IL-13R alpha. The secreted extracellular domain of IL-4R alpha, called sIL-4R alpha, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naïve CD4+ T cells and class switching to IgG1 and IgE. Recombinant Human sIL-4 Receptor alpha is a 23.9 kDa glycoprotein corresponding to 209 amino acid residues of the extracellular domain of IL-4R alpha. As a result of glycosylation, Recombinant Human sIL-4 Receptor alpha migrates with an apparent molecular mass of approximately 50-65 kDa by SDS-PAGE gel, under reducing conditions.
Protein SequenceGNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH
Uniprot IDP24394
Protein RefSeqNP_000409.1
mRNA RefSeqNM_000418

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