Mouse Podoplanin, soluble

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Cat-Nr.S01-M46
Size5 µg
Price70 €
SourceE. coli
LabelHis-Tag
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & visualized by silver stain
Length [aa]126
N Terminal SequenceGTIGVNEDDIVTPGT
Biological ActivityTesting in progress.
BufferPBS
ReconstitutionWe recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
Stability and StorageThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted sPodoplanin should be stored in working aliquots at -20 °C.
SynonymsPdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
DescriptionPodoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Protein SequenceGTIGVNEDDIVTPGTGDGMVPPGIEDKITTTGATGGLNESTGKAPLVPTQRERGTKPPLEELSTSATSDHDHREHESTTTVKVVTSHSVDKKTSHPNRDNAGDETQTTDKKDGLPVVTLEHHHHHH
Uniprot IDQ62011
Protein RefSeqNP_034459.2
mRNA RefSeqNM_010329.2

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