Human CD4, soluble

Slide this table

Cat-Nr.S01-008
Size50 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation >95% by SDS-PAGE & HPLC analysis
Length [aa]371
Molecular Weight41.3 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityDetermined by its ability to binds HeLa cellss expressing cell-surface MHC-II molecules.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
SynonymsCluster determinant 4, T-cell surface glycoprotein CD4, Interleukin IL-16 receptor, T-cell surface antigen T4/Leu-3, Leu-3(a, b), OKT4(a-f), L3T4 (mouse)
DescriptionCluster determinant 4 (CD4), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, playsan integral role in signal transduction and T-cell differentiation, development and activation. CD4 is constitutively expressed on thesurface of various immune cells, including monocytes, macrophages, eosinophils, dendritic cells, and most prominently Tlymphocytes,where it functions as an essential co-receptor and co-ligand for T-cell receptor (TCR) and major histocompatibilitycomplex class II (MHC-II) molecules. Ligation by MHC-II molecules on the surface of antigen-presenting cells can serve toinfluence adaptive immunity by facilitating helper T-cell activation and macrophage differentiation, while ligation by proinflammatorycytokine IL-16 can contribute to innate immunity by chemoattracting CD4-expressing peripheral immune cells alongan IL-16 gradient for their recruitment and activation at sites of inflammation. The protean functionality of CD4 extends pastimmunity as CD4 also notably serves as the major receptor for HIV-1 and human herpes virus 7 (HHV-7) infections. During HIVpathogenesis, CD4 acts instrumentally as a high-affinity entry receptor for the internalization of HIV-1 following binding of the viralenvelope glycoprotein gp120 to CD4’s extracellular domain. Recombinant Human sCD4 is amonomeric glycoprotein of 371 amino acid residues, which correspond to the extracellular CD4 domain, and a calculated molecularweight of 41.3 kDa. As a result of glycosylation, Recombinant Human sCD4 migrates with an apparent molecular mass of approximately 45-55 kDa by SDS-Page Gel analysis, under reducing conditions.
Protein SequenceKKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LK
Uniprot IDP06332
Protein RefSeqNP_038516.1
mRNA RefSeqNM_013488.2

All prices plus VAT + possible delivery charges