Human MIF

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Cat-Nr.100-418
Size25 µg
Price180 €
SourceInsect cells
LabelHis-Tag
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]124
Molecular Weight15 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityDetermined by its ability to inhibit monocyte migration.
Species ReactivityHuman
Buffer10mM Sodium Citrate, pH 3.0
ReconstitutionCentrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Stability and StorageThe lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
SynonymsMacrophage migration Inhibitory factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor
DescriptionMacrophage Migration Inhibitory Factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-α, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. Insect cell derived recombinant MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
Protein SequenceHHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA
Uniprot IDP14174
Protein RefSeqNP_002406.1
mRNA RefSeqNM_002415.1

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