Rabbit Anti-Human Podoplanin

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Cat-Nr.102-PA40
Size200 µg
Price290 €
CategoryPolyclonal Antibody
Clone Nr.Rabbit IG
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C. Avoid repeated freeze-thaw cycles!
PreparationProduced from sera of rabbits immunized with the recombinant ectodomain of Podoplanin (gp36). Podoplanin is a highly O-glycosylated integral membrane protein that is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, Podoplanin colocalizes with VEGFR-3/FLT-4, another marker for lymphatic endothelial cells.
AntigenRecombinant human Podoplanin (RT #S01-046)
ApplicationWB, IHC, IP
SynonymsPDPN; T1A; GP36; GP40; Gp38; OTS8; T1A-2; AGGRUS; HT1A-1; PA2.26
DescriptionPodoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Uniprot IDQ86YL7
Protein RefSeqNP_001006625.1
mRNA RefSeqNM_001006624.1

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