Rabbit Anti-Human ccbe1
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| Cat-Nr. | 102-PA36 |
| Size | 200 µg |
| Price | 290 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | |
| Antigen | Highly pure (>98%) recombinant human ccbe1 (Cys159-Leu251) derived from E. coli. |
| Application | WB, IF |
| Synonyms | collagen and calcium binding EGF domains 1; KIAA1983 |
| Description | The lymphatic system comprises a vascular system separate from the cardiovascular system, essential for immune responses, fluid homeostasis and fat absorption. Lymphatic vessels develop in a complex process termed lymphangiogenesis that involves budding, migration and proliferation of lymphatic endothelial progenitor cells. A few genes, such as FLT4, FOXC2 and SOX18, are known to be critically involved in lymph vessel formation in humans. Lymphedema, lymphangiectasias, mental retardation and unusual facial characteristics define the autosomal recessive Hennekam syndrome. Homozygosity mapping identified a critical chromosomal region containing ccbe1, encoding Collagen and Calcium-Binding EGF-domain-1, a secreted protein which is required for embryonic lymphangiogenesis in zebrafish. ccbe1 is not expressed in endothelial cells of lymph vessels, and it may be a component of the extracellular matrix. In zebrafish, ccbe1 expression was observed along the earliest migration routes of endothelial cells that sprout from the posterior cardinal vein and migrate circuitously before developing into lymphatic vessels. ccbe1 might therefore be involved in guidance of these migrating cells. |
| Uniprot ID | Q6UXH8 |
| Protein RefSeq | NP_597716.1 |
| mRNA RefSeq | NM_133459.3 |
Figures
Immunofluorescence staining of ccbe1 (red) in human foreskin tissue using a polyclonal rabbit anti-human ccbe1 antibody [Cat# 102-PA36] and counter staining of nuclei with Dapi. The section was fixed with 4% PFA for 25 min, the antibody was diluted 1:100. [B] Control without primary antibody (yellow in A and green in B correponds to the autofluorescence within the Membrana elastica interna of an artery). A signal is visible in fibrocytes, smooth muscle cells and probably in endothelial cells.
The IF experiments were performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
Immunoperoxidase staining of ccbe1 in human skin using a polyclonal rabbit anti-human ccbe1 antibody [Cat# 102-PA36]. The section was fixed with 4% PFA overnight, the antibody was diluted 1:100. [B] Control without primary antibody. A signal is visible in smooth muscle cells, a little bit weaker in endothelial cells as well as in the connective tissue.
The IF experiments were performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
Immunofluorescence staining of ccbe1 (red) in human placenta tissue using a polyclonal rabbit anti-human ccbe1 antibody [Cat# 102-PA36]. The section was fixed with 4% PFA for 25 min, the antibody was diluted 1:100. [B] Control without primary antibody. A signal is visible in fibrocytes, smooth muscle cells and probably in endothelial cells.
The IF experiments were performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
Western Blot Analysis of anti-human ccbe1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa); lane 2: rh ccbe1.
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