Rabbit Anti-Human ESAM

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Cat-Nr.102-PA42S
Size100 µg
Price175 €
CategoryPolyclonal Antibody
Clone Nr.Rabbit IG
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute in sterile water to a concentration of 0.1-1.0mg/mL.
Stability and StorageThe lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C. Avoid repeated freeze-thaw cycles!
PreparationProduced from sera of rabbits immunized with highly pure recombinant human soluble ESAM (Endothelial cell-selective adhesion molecule; Q30-A247; SwissProt: #Q96AP7) as the immunizing antigen. Anti-human ESAM was purified by affinity chromatography with immobilized Protein A.
AntigenRecombinant human ESAM (RT #300-057)
ApplicationWB, IF; FC
SynonymsESAM; W117m
DescriptionEndothelial cell selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in hemophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI1. The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF2.
Uniprot IDQ96AP7
Protein RefSeqNP_620411.2
mRNA RefSeqNM_138961.2

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