Mouse Anti-Human Ret

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Cat-Nr.101-M619
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#10F12)
IsotypeIgG1
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with extracellular domain of recombinant human Ret.
Antigenrecombinant human Ret extracellular domain
ApplicationWB
SynonymsRET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
DescriptionThe GDNF family of neurotrophic factors forms a subfamily within the TGFβ superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosylphosphatidylinositol (GPI)linked ligandbinding subunits (GFRα14). GFRα1 2, 3 and 4 are the preferred ligandbinding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosinekinase receptor is encoded by the c-ret protooncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNFGFRα complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFRα.
Uniprot IDQ9BTX6
Protein RefSeqNP_066124.1
mRNA RefSeqNM_020975.4

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