Mouse Anti-Human TNF-alpha

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Cat-Nr.101-M17
Size500 µg
Price250 €
CategoryMonoclonal Antibody
IsotypeIgG1, kappa
Species ReactivityHuman
Formulationlyophilized from PBS
BufferPBS
ReconstitutionReconstitute the antibody in sterile water to a concentration of 1.0mh/mL.
Stability and StorageThe lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.
PreparationProduced in BALB/c mice using highly pure (>98%) recombinant human TNF-alpha as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
AntigenRecombinant human TNF-alpha
ApplicationELISA, WB, IHC (P), N
SynonymsTNF; DIF; TNFA; TNFSF2; TNF-alpha
DescriptionTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.
Uniprot IDP01375
Protein RefSeqNP_000585.2
mRNA RefSeqNM_000594

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