Rat Anti-Mouse Sonic Hedgehog (Shh) C-terminal

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Cat-Nr.103-M269
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#4A37)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant Sonic Hedgehog (Shh) C-Terminal fragment. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigenrecombinant mouse Shh (C-terminal) fragment
ApplicationWB
SynonymsShh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
DescriptionHuman Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage.
Uniprot IDQ62226
Protein RefSeqNP_033196.1
mRNA RefSeqNM_009170.3

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