Rat Anti-Human Relaxin-1

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Cat-Nr.101-M616
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#6L12)
IsotypeIgG2
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with recombinant human Relaxin-1.
Antigenrecombinant human Relaxin-1
ApplicationWB, N
SynonymsRLN1; H1; RLXH1; bA12D24.3.1; bA12D24.3.2
DescriptionHuman Relaxin1, also called H1 Relaxin or RLN1, is one of three human relaxins in the structurally related insulin/relaxin superfamily. Relaxin1 is thought to be the result of duplication of the Relaxin2 gene in higher primates only. In species below higher primates, Relaxin1 is the equivalent of human Relaxin2. Relaxin1 is found in some but not all tissues expressing Relaxin2. It is prominent in the prostate, but also present in decidua, placenta, endometrium and at low levels in the myocardium. As with other insulin/relaxin superfamily members, human Relaxin1 is synthesized as a preprohormone. Processing of the 21 kDa preprorelaxin1 includes removal of the signal sequence, formation of two disulfide bonds between A and B chains and removal of the intervening C-chain by a prohormone convertase. The resulting mature protein is an unglycosylated, 6 kDa dimer of disulfidelinked A and B chains. Human Relaxin1 shares 76% amino acid (aa) identity with human Relaxin2, and 43%, 50% and 43% aa identity with mouse, rat and canine Relaxin1, respectively. An alternate splice form of unknown significance has a 47 aa substitution which does not have typical Cchain cleavage motifs. Relaxins confer activity by binding to leucinerich G-protein coupled receptors LGR7 and LGR8. Prostatic relaxins are antiapoptotic and contribute to development and maintenance of male fertility. It is not clear whether human Relaxins 1 and 2 have distinct functions. Both use the same receptor and have the same critical amino acids for folding and for receptor interaction. While receptor affinity is similar, activity is lower for Relaxin1 as compared to Relaxin2. Progesterone increases expression of only Relaxin2, while glucocorticoids increase expression of both.
Uniprot IDP04808
Protein RefSeqNP_008842.1
mRNA RefSeqNM_006911.2

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