Mouse Anti-Human CEACAM-1
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|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Stability and Storage||The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.|
|Preparation||Monoclonal antibodies were produced with the help of BALB/c mice using recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells) as the immunizing antigen. The antibody (clone #B3) was purified from cell culture supernatant by Protein G chromatography.|
|Antigen||recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)|
|Application||WB, IHC, E, IF, IP, FC|
|Synonyms||CEACAM1; BGP; BGP1; BGPI, CD66a|
|Description||Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, it forms noncovalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist.|
- Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells. E. Klaile et al., mBio. 2017 Mar-Apr; 8(2): e02142-16.
- CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production. V. Khairnar et al., Nat Commun. 2015 Feb 18; 6: 6217.
- Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions. M. Seifert et al., Proc Natl Acad Sci U S A. 2015 Feb 10; 112(6): E546–E555.
- Soluble CEACAM8 Interacts with CEACAM1 Inhibiting TLR2-Triggered Immune Responses. B. B. Singer et al., PLoS One. 2014; 9(4): e94106.
- Tumor and Endothelial Cell-Derived Microvesicles Carry Distinct CEACAMs and Influence T-Cell Behavior. H. T. Muturi et al., PLoS One. 2013; 8(9): e74654.
- Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons. E. Klaile et al., Respir Res. 2013; 14(1): 85.
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