Rat Anti-Mouse P-Selectin

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Cat-Nr.103-M457
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#17H23)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of P-Selectin EC domain.
Antigenrecombinant mouse P-Selectin extracellular domain
ApplicationWB
SynonymsSelp; Grmp; CD62P; LECAM3; PADGEM; GMP-140
DescriptionMouse -P-Selectin (GMP140, LECAM3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P- Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or WeibelPalade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL 1 (PSelectin glycoprotein ligand1). PSelectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with LSelectin, PSelectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving ESelectin, and later ICAM1 and VCAM1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2t-erminal C-type lectin domain and an EGF like domain followedby a series of of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.
Uniprot IDQ01102
Protein RefSeqNP_035477.1
mRNA RefSeqNM_011347.2

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