Rat Anti-Mouse TIMP-1

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Cat-Nr.103-M277
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#6A34)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TIMP-1. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigenmouse recombinant TIMP-1 protein
ApplicationWB
SynonymsTimp1; Clgi; Timp; TIMP-1
DescriptionTIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
Uniprot IDP12032
Protein RefSeqNP_035723.2
mRNA RefSeqNM_011593.2

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