Rat Anti-Mouse TSG (Twisted gastrulation)

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Cat-Nr.103-M281
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#7D19)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TSG. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigen Mouse recombinant protein of TSG
ApplicationWB, IHC (P)
SynonymsTwsg1; Tsg; Twg; AW552143; D17Ertd403e; 1810013J15Rik; 9030422N06Rik
DescriptionTwisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. TSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.
Uniprot IDQ9EP52
Protein RefSeqNP_075540.1
mRNA RefSeqNM_023053.3

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