Mouse Anti-Human Podocalyxin

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Cat-Nr.101-M142
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#GB6D9C11)
IsotypeIgG1
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.
PreparationMonoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated human glomeruli as the immunizing antigen. The mouse IgG1 antibody (#4D5aE5E6) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
Antigenmembrane protein fraction of isolated human glomeruli
ApplicationIHC (P); IF
SynonymsPODXL; PC; PCLP; Gp200; PCLP-1; Podocalyxin-like protein, GCTM-2 antigen, Gp200
DescriptionPodocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. It was shown that endothelial cell membranes contain Podocalyxin. By immunoelectron microscopy Podocalyxin was localized on the luminal membrane domain of endothelial cells, in a patchy distribution. The presence of Podocalyxin was confirmed in SDS extracts of lung tissue by immunoblotting. It is concluded that (a) Podocalyxin is a widespread component of endothelial plasma membranes, (b) it is restricted to the luminal membrane domain, and (c) it is distributed unevenly on the endothelial cell surface.
Uniprot IDO00592
Protein RefSeqNP_001018121
mRNA RefSeqNM_001018111

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