The purification of a recombinant protein is the most complex step in the whole service cascade. While cloning of a gene into a transfer vector is often highly dependent on the cDNA fragment size, an efficient purification procedure depends on the biochemical characteristics of the individual protein (e.g. amino acid composition, IP value, glycosylation, stability). In additon individual features of the protein (e.g. secreted, cytoplasmatic or membrane-bound protein, mono- or oligo- or polymeric protein) must be take into account establishing an efficient purification strategy.
Adding a “tag” to a protein (either N- or C- terminally) strongly supports the purification or at least the enrichment of the protein applying standard purification protocols (e.g. using protein A or G Sepharose for the Fc-tag). In addition the broad commercial availability of “tag” - specific antibodies allows an excellent fallback option for protein detection as well as for monitoring all purification steps if antibodies for the specific protein of interest are not available yet.
With step E of our protein production services in insect cells we support you to design and establish the optimal strategy to purify your protein of interest.
Note: This part is offered only in combination with step E and a recombinant protein containing a “tag”! For purification of recombinant proteins without an established purification protocol the price will depend on the required time and material needed!
Cell pellet or supernatant from service E.
Dependent on the volume ordered.