Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF
> 98% by SDS-PAGE & HPLC analyses
Determined by its ability to increase IFN-γ production by anti-TCR mAb-stimulated PBMCs.
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p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKAR
QTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET
SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLM
DPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK
IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKD
VYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KT
LTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQ
KE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDP
QGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVM
V DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSW
EYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK
NASISVRAQD RYYSSSWSEW ASVPCS
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 p70, derived from HEK293 cells, is a heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits (503 total amino acid residues). The calculated molecular weight of Human IL-12 p70 is 57.2 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of 65-70 kDa based on SDS-PAGE gel, under non-reducing conditions.
NCBI Gene ID: