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Protocols for IHC from Researchers
  

The following protocols are form researchers using RELIATech's antibodies:

1. Anti-mouse LYVE-1   
a) For cryo sections (Cat# 103-PA50) (PDF)
b) For whole mount histology (Cat# 103-PA50) (PDF)
c) For paraffin-embedded sections (Cat# 103-PA50AG) (PDF)
d) For cryo sections of rat tissue (Cat# 103-PA50S) (PDF)

2. Anti-human LYVE-1
a) For cryo sections (Cat# 102-PA50) (PDF)
b) For paraffin-embedded sections (Cat# 102-PA50AG) (PDF)
c) For paraffin-embedded sections II (Cat# 102-PA50AG) (PDF)
d) For paraffin-embedded sections III (Cat# 102-PA50S/-PA50) (PDF)

3. Anti-mouse Podoplanin (Cat# 103-M40)
a) For paraffin-embedded sections (PDF)

4. Anti-human Podoplanin (Cat# 101-M40)
a) For paraffin-embedded sections (German, PDF)
b) For paraffin-embedded sections (English, PDF)
c) For paraffin-embedded sections, Cat# 101-M41 (PDF)

5. Anti-rat Podoplanin (Cat# 104-M40)
a) For paraffin-embedded sections (PDF)

6. Anti-human Podoplanin (Cat# 102-PA40)
a) For paraffin-embedded sections (German, PDF)
b) For paraffin-embedded sections (English, PDF)

7. Anti-Prox-1 (Cat# 102-PA30)
a) For paraffin-embedded sections (PDF)

8. Anti-Prox-1 (Cat# 102-PA32AG)
a) For paraffin-embedded sections from equine ceek teeth (PDF, German)
b) For paraffin-embedded sections from equine ceek teeth (PDF, Engl)

9. Anti-human TIE-2/tek (Cat# 101-M50 /-M52 /-M54)
a) For cryo sections (PDF)

10. Protocol for immunohistochemical staining
 a) For frozen sections (PDF)
 
11. Anti-mouse LEC and BEC
 a) For acetone or paraformaldehyde fixed sections (PDF)

12. Anti-human Podocalyxin (Cat# 101-M142)
a) For paraffin-embedded sections (PDF)

13. Anti-human Megalin (Cat# 101-M144)
a) For paraffin-embedded sections (PDF)

14. Anti-rat Aminopeptidase P (Cat# 104-M01)
a) For paraffin-embedded sections (PDF)

15. Anti-mouse CD4 (#2H9) (Cat# 103-M105)
a) For paraffin-embedded sections (PDF)

16. Anti-mouse CD8a (#10F14) (Cat# 103-M106)
a) For paraffin-embedded sections (PDF)


Immunofluorescence with LEC!
  

a) Protocol for immunofluorescence with lympathic endothelial cells (LEC) using Lab-Tek II Chamber Slides. As primary antibodies anti-human LYVE-1 (RELIATech Cat# 102-PA50), anti-human Prox-1 (RELIATech Cat# 102-PA32) and anti-human CD31were used (PDF).

b) Protocol for immunofluorescence with lympathic endothelial cells (LEC) and HUVECs in a cell culture dish. As primary antibodies anti-human Prox-1 (RELIATech Cat# 102-PA32) and anti-human CD31were used (PDF).

FACS analysis
  

FACS analysis with human macro- / microvascular endothelial cells.

a) HUVEC / HDMVEC (German / English)

 

Bioassays
  

Thymidine incorporation assay for primary human endothelial cells (PDF)

 

Thymidine incorporation assay for Balb/c 3T3 cells (German / English

 

Demasking of epitops in Paraffin-embedded sections!
  

Demaskierung von Epitopen im Paraffinschnitt

Gewebefixierung und -vorbereitung (PDF)
Target Retrieval Puffer (
PDF)

Hitzebehandlungen

- im Autoklav (PDF)
- im Mikrowellengerät (
PDF)
- im Schnellkochtopf (
PDF)
- im Wasserbad (
PDF)
- mit Wasserdampf (
PDF)

Proteolytische Vorbehandlung (PDF)
Kombinantion von Methoden (
PDF)

 

Biologo
  

Demaskierung von Antigenen für IHC

Die Firma biologo bietet verschiedene Pufferlösungen für die Demaskierung von Antigenen nach Aldehydfixierung an. Demaskierungslösung G basiert auf Glykol-Basis, bei Demaskierungslösung E handelt es sich um einen EDTA-Puffer. 

Demasking of antigens for IHC

biologo a German biotech company offers different buffer solutions for demasking of antigens after fixation with formaldehyde. Demasking solution G is based on Glycol, demasking solution E is a EDTA buffer.

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Updated 23.08.2010   

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