Figures

Figure 1.
Immunohistochemistry with paraffin-embedded sections of mouse intestine using a polyclonal rabbit anti-mouse [Cat# 103-PA50AG] LYVE-1 antibody. You see the staining (red) of lymphatic endothelial cells of the intestine.
The experiment was performed by Dr. Karsten Debel, DCS, Hamburg, Germany.
Figure 2.
Immunohistochemical analysis of a healthy mouse heart using ReliaTech's rabbit anti-mouse LYVE-1 antibody [Cat# 103-PA50AG]. Heart was labeled with polyclonal rabbit anti-mouse LYVE-1 (1:500 dilution (corresponds 0.4 μg/ml)) followed by 1:400 dilution of a Cy5-conjugated donkey anti-rabbit secondary antibody (Jackson). Extensive washing was performed to remove nonspecific binding. Hearts were clarified using a modified iDISCO+ protocol as described in "Arterioscler Thromb Vasc Biol. 2020 May 14:ATVBAHA120314370. doi: 10.1161/ATVBAHA.120.314370.“ Evaluation by lightsheet microscopy (ultramicroscope II, x0.8, scale bar 1 mm) of a whole mount-stained mouse heart reveals a dense lymphatic network (Lyve1, blue) on the epicardial surface.
The experiment was performed by the research group of Dr. Hab. Ebba Brakenhielm, Inserm U1096 EnVI Laboratory, Medical Faculty of Rouen University, and David Godefroy, Inserm U1245 (DC2N Laboratory), Mont Saint Aignan, France.
Figure 3.
Rat cardiac lymphatic microvessels labeled with antibodies against mouse LYVE-1 (red), and adjacent blood vessels, labeled with antibodies against CD31 (green). Nuclear stain in blue. Scale bar = 50 µm.
Note: The anti-mouse Lyve-1 polyclonal antibody (Cat# 103-PA50AG) shows a strong cross reaction with rat LYVE-1 protein.
The experiment was performed by the research group INSERM U1096 in Rouen, France directed by Dr Vincent Richard.
Figure 4.
Staining of CD31 (blood vessel - green) showing capillary structures and visualized using whole mount histology. Overlaid staining for LYVE-1 (lympatic vessel - red) using a polyclonal rabbit anti-mouse LYVE-1 antibody [Cat# 103-PA50AG].
The experiments were performed by Scott Gerber & Edith Lord, PhD, University of Rochester, USA
Figure 5.
Immunofluorescence staining (red) of cryo-sections of mouse skin day 17.5) (upper panel) and human foreskin (lower panel) with anti-mouse LYVE-1 (5µg/ml) [Cat# 103-PA50AG] and counter staining of nuclei with Dapi (blue).
The experiments were performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
Figure 6.
FACS analysis with primary mouse lung microvascular endothelial cells (MLMEC).

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