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Figure 1.
Cryo sections of mouse colon carcinoma labeled with rabbit polyclonal antibody against mouse LYVE-1 (red) [Cat# 103-PA50] and human CD31 (green). A: CD31; B: LYVE-1; C: CD31/LYVE-1
The experiments were performed by Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research Tumor Biology Center, Breisacher Str. 117, D-79106 Freiburg, Germany
Figure 2.
Immunohistochemistry with paraffin-embedded sections of mouse intestine using a polyclonal rabbit anti-mouse [Cat# 103-PA50] LYVE-1 antibody. You see the staining (red) of lymphatic endothelial cells of the intestine.
The experiments has been performed by Dr. Karsten Debel, DCS, Hamburg, Germany.
Figure 3.
Rat cardiac lymphatic microvessels, labeled with an antibody against rat Podoplanin [Cat# 104-M40] (left panel) and a antibody against mouse LYVE-1 (right panel). Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 μm. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) / mouse anti-Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.
The experiments were performed by the research group of Prof. Dr. E. Brakenhielm – Rouen University (see also: Henri O et al., Circulation, March 2016).
Figure 4.
LYVE1/CD31 staining on frozen sections of the mouse prostate.
The experiments were performed by Scott Gerber & Edith Lord, PhD, University of Rochester, USA
Figure 5.
Western Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
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