Protocols from Researchers

The following protocols are from researchers using ReliaTech's antibodies.

Immunohistochemistry (IHC)

Epitope demasking

Demasking of epitopes in paraffin sections

Heat treatments

Proteolytic Treatments

Proteolytische Vorbehandlung (PDF)
Kombinantion von Methoden (PDF)

Immunofluorescence (IF)

Immunofluorescence with lymphatic endothelial cells (LECs)

a) Protocol for immunofluorescence with lympathic endothelial cells (LEC) using Lab-Tek II Chamber Slides. As primary antibodies anti-human LYVE-1 (ReliaTech Cat# 102-PA50), anti-human Prox-1 (ReliaTech Cat# 102-PA32) and anti-human CD31 were used (PDF, English).

b) Protocol for immunofluorescence with lympathic endothelial cells (LEC) and HUVECs in a cell culture dish. As primary antibodies anti-human Prox-1 (ReliaTech Cat# 102-PA32) and anti-human CD31 were used (PDF, English).

 

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Flow Cytometry

FACS analysis with human macro- / microvascular endothelial cells

a) HUVEC / HDMVEC (German / English)

Bioassays

a) Thymidine incorporation assay for primary human endothelial cells (PDF, English)

b) Thymidine incorporation assay for Balb/c 3T3 cells (German / English)

 

Protocols for ReliaTech Antibodies

Protocols for ReliaTech Antibodies

1. Anti-mouse LYVE-1   
a) For cryo sections (Cat# 103-PA50) (PDF)
b) For whole mount histology (Cat# 103-PA50) (PDF)
c) For paraffin-embedded sections (Cat# 103-PA50AG) (PDF)
d) For cryo sections of rat tissue (Cat# 103-PA50S) (PDF)

2. Anti-human LYVE-1
a) For cryo sections (Cat# 102-PA50) (PDF)
b) For paraffin-embedded sections (Cat# 102-PA50AG) (PDF)
c) For paraffin-embedded sections II (Cat# 102-PA50AG) (PDF)
d) For paraffin-embedded sections III (Cat# 102-PA50S/-PA50) (PDF)

3. Anti-mouse Podoplanin (Cat# 103-M40)
a) For paraffin-embedded sections (PDF)

4. Anti-human Podoplanin (Cat# 101-M40)
a) For paraffin-embedded sections (German, PDF)
b) For paraffin-embedded sections (English, PDF)
c) For paraffin-embedded sections, Cat# 101-M41 (PDF)

5. Anti-rat Podoplanin (Cat# 104-M40)
a) For paraffin-embedded sections (PDF)

6. Anti-human Podoplanin (Cat# 102-PA40)
a) For paraffin-embedded sections (German, PDF)
b) For paraffin-embedded sections (English, PDF)

7. Anti-Prox-1 (Cat# 102-PA30)
a) For paraffin-embedded sections (PDF)

8. Anti-Prox-1 (Cat# 102-PA32AG)
a) For paraffin-embedded sections from equine cheek teeth (PDF, German)
b) For paraffin-embedded sections from equine cheek teeth (PDF, Engl)

9. Anti-human TIE-2/tek (Cat# 101-M50 /-M52 /-M54)
a) For cryo sections (PDF)

10. Protocol for immunohistochemical staining
 a) For frozen sections (PDF)
 
11. Anti-mouse LEC and BEC
 a) For acetone or paraformaldehyde fixed sections (PDF)

12. Anti-human Podocalyxin (Cat# 101-M142)
a) For paraffin-embedded sections (PDF)

13. Anti-human Megalin (Cat# 101-M144)
a) For paraffin-embedded sections (PDF)

14. Anti-rat Aminopeptidase P (Cat# 104-M01)
a) For paraffin-embedded sections (PDF)

15. Anti-mouse CD4 (#2H9) (Cat# 103-M105)
a) For paraffin-embedded sections (PDF)

16. Anti-mouse CD8a (#10F14) (Cat# 103-M106)
a) For paraffin-embedded sections (PDF)