High titer stock production of a recombinant baculovirus

Material we need from you:

2 ml of recombinant virus stock, preferably with a known viral titer. The sample is either provided by you or prepared by us if Service B has been performed.

Our service comprises:

  • Infection of 0.75x108 insect cells and harvesting of supernatant at 72 h post- infection (100 ml virus stock!).

Note: Upon customer request, this service can be performed with or without serum!

  • Viral titer determination of the viral stock using plaque assay.

 

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Results provided to you:

  • 100 ml of recombinant virus stock at about 5x107 to 1x108 pfu/ml.
  • A detailed report sheet.

Expected time range:

3–4 weeks.