Service B: Generation of a recombinant E. coli strain

This step enables you to produce your own recombinant E. coli strain for your individual production purposes.

Material we need from you:

10-30 μg purified and characterized E. coli expression vector DNA bearing the gene to be expressed. The vector is either provided by you or prepared by us (for further information concerning our cloning services, please click here).

Note: For the optimal performance of a new protein it is recommended to test the bacterial host by using several different E.coli strains for the analytical production, e.g. BL21 (DE3), BL21 Star (DE3), Rosetta (DE3) and others (please, inquire).

Our service comprises:

  • Generation of a recombinant E.coli strain using standard protocols.
  • Isolation and cultivation of 6 single clones over night.
  • Analytic production (1ml scale)
  • SDS-PAGE and subsequent Coomassie stain of total lysate from induced/non-induced samples.

 

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The induction of our standard constructs for protein expression is performed by temperature shift or IPTG.

Expected time range:

1-3 weeks