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    Human Follistatin like-protein 1/FSTL1 NEW

    Host:
    CHO cells
    Size:
    50 µg
    Price:
    € 170
    Formulation:
    lyophilized
    Catalog Number:
    100-437
    Synonyms:
    Follistatin-like protein 1, FSL1, Follistatin-related protein 1, FRP, OCC1, OCC-1, TGF-β1-stimulated clone 36, TSC-36
    Purity Confirmation:
    > 95% by SDS-PAGE & HPLC analyses
    Biological activity:
    Measured by its binding ability in a functional ELISA. When recombinant human Follistatin like 1/FSTL1 is coated at 200 ng/well, the concentration of recombinant human BMP-4 that produces 50% of the optimal binding response is found to be approximately 250 ng/mL.
    Protein Sequence:
    EEELRSKSKI CANVFCGAGR ECAVTEKGEP TCLCIEQCKP HKRPVC GSNG KTYLNHCELH RDACLTGSKI QVDYDGHCKE KKSVSPSASP V VCYQSNRDE LRRRIIQWLE AEIIPDGWFS KGSNYSEILD KYFKNFD NGD SRLDSSEFLK FVEQNETAIN ITTYPDQENN KLLRGLCVDA LI ELSDENAD WKLSFQEFLK CLNPSFNPPE KKCALEDETY ADGAETEV DC NRCVCACGNW VCTAMTCDGK NQKGAQTQTE EEMTRYVQEL QKH QETAEKT KRVSTKEI
    Description:
    Follistatin-like protein 1 (FSTL1) is a widely-expressed, extracellular glycoprotein that is homologously grouped into the osteonectin (BM-40/SPARC) family of secreted proteins based on its possession of both a follistatin-like and extracellular calcium-binding domain. Initially identified as a TGF-beta-inducible protein in a cloned mouse osteoblast cell line, FSTL1 has since been implicated in an array of cell-type-specific functions, such as the regulation of proliferation, differentiation, apoptosis and migration, as well as a number of biological processes, including embryonic development, inflammatory response, angiogenesis, tumorigenesis, and immune disease pathogenesis. Highly conserved across mammalian species and widely expressed in human tissues, FSTL1 can be upregulated through signaling mediators of the innate immune system, such as TLR4 agonists and the arthritogenic cytokine IL-1beta via NFκB pathways, to stimulate the expression and secretion of pro-inflammatory cytokines, including TNF-alpha, IL-1beta, IL-6 and IL-8. While cells of mesenchymal lineage are capable of FSTL1 production, FSTL1 expression is notably absent from cells of hematopoietic lineage under normal physiological conditions. Macrophages and monocytes are, however, capable of taking up FSTL1 at sites of inflammation where FSTL1 stimulation can cause the expression of caspase-1 and its resultant enzymatic cleavage of active IL-1beta from pro-IL-1beta. Whereas the overexpression of FSTL1 has been noted as a substantial contributor to the progression of immune diseases like rheumatoid arthritis (RA) and osteoarthritis (OA), diminished FSTL1 serum levels have been identified as playing a significant part in both ovarian and endometrial carcinogenesis, where it directly affects cell proliferation, migration and invasion. The CHO cell-derived recombinant human FSTL1 is a 288-amino-acid-length glycoprotein with a calculated molecular weight of 32.7 kDa; however, due to glycosylation, protein migration occurs at an apparent molecular weight of approximately 50-55 kDa by SDS-PAGE analysis under both reducing and non-reducing conditions.
    NCBI Gene ID:
    11167
    Uniprot:
    Q12841
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